School Science Lessons
2024-05-30

Tests for all substances
(UNChemTests) Contents
Confirmation tests with original solution or solid: 12.11.3.10
Electrical inspections tests: 4.2.4
GroupTests, Chemical Tests
Tests for
Tests with multiple reagent strips: 19.1.20.5
Solubility table: 7.7.1
Solubility rules: 7.7.3
Test reagents: 9.7.15 (commercially available)


Tests for all substances
Tests for acetates: 12.11.5.1
Tests for acetic acid in vinegar: 12.11.3
Tests for acetylene (ethyne): 16.1.3.2
Tests for acid-base indicators: 3.53.1
Tests for acid radicals in solution: 12.11.2
Tests for air and dissolved oxygen in water: 18.3.0a
Tests for air pollution from burning refuse: 18.6.1
Tests for albumin and gelatine: 9.6.4
Tests for alcohols, potassium manganate (VII): 16.1.3.1.1a
Tests for aldehydes with Fehling's solution: 9.142.2
Tests for aldehydes, Tollens' test, silver mirror: 9.3.7.3
Tests for aluminium: 12.0.2
Tests for aluminium compounds: 12.1.11
Tests for aluminium compounds in solution: 12.1.12
Tests for ammonia or amino acids or proteins acids with ninhydrin: 10.2.9
Tests for ammonia: 3.5.0
Tests for amylose and amylopectin: 9.132a
Tests for anions: 12.11.0
Tests for antimonates, borates, oxalates: 12.11.2a
Tests for antimony: 12.11.3.12
Tests for arsenates: 12.11.3
Tests for arsenic: Heat with charcoal, light blue flame test if moistened with hydrochloric acid.
Tests for arsenic, flame tests: 12.11.7.2b (See: Arsenic)
Tests for ash content of plant dry matter: 9.3.16
Tests for aspirin: 12.1.9, C9H8O4
Tests for barium: 12.11.3.13
Tests for bicarbonates: 12.11.4
Tests for carbonates / bicarbonates: 12.11.7
Tests for bismuth: 12.11.3.14
Tests for bismuth: 12.11.3.8, (See: 3.)
Tests for blood: 9.9.1
Tests for borates: 12.11.5
Tests for borax / turmeric adulteration of food: 19.1.32
Tests for breakdown of starch to sugars: 9.5.11
Tests for bromides: 12.11.6
Tests for cadmium: 12.11.3.8, (See: Heat with charcoal 4.)
Tests for cadmium: 12.11.3.15
Tests for calcium: 16.5.5
Tests for calcium, flame test: 12.11.3.16
Tests for calcium carbonate (limestone): 35.31 (Geology)
Tests for carbohydrates, Molisch's test: 9.134
Tests for carbon dioxide: 3.1.0
Tests for carbon monoxide with a gas detector: 18.6.3.1
Tests for carbonates / bicarbonates: 12.11.7
Tests for carbonates: 35.13, effervescence test, hydrochloric acid test, (Geology)
Tests for cellulose, iodine test: 9.135
Tests for cellulose, solubility test: 9.136
Tests for cement brick strength (contents): 34.2.3
Tests for cement brick strength (water content): 34.2.4
Tests for cement change in weight when setting: 34.2.5
Tests for chewing gum quality by comparing bubbles: 16.7.30
Tests for chlorides: 12.11.8
Tests for chlorine 1: 15.5.12a
Tests for chlorine 2: 3.40.1
Tests for chlorine 3: 18.7.21.0, (Test kit for swimming pools)
Tests for chlorine 4: 18.7.4.0, (Tests for free chlorine in water, swimming pools)
Tests for chromates: 12.11.9
Tests for chromium: 12.11.3.17
Tests for cigarette smoke: 16.5.6
Tests for cobalt: 12.11.3.18
Tests for common solutions with acid-base indicators: 5.6.2
Tests for concrete alkalinity: 34.66.3
Tests for copper
Tests for dextrins in toast: 19.3.4.1
Tests for diastase activity: 9.3.10
Tests for dinitrogen oxide, nitrous oxide, N2O: 3.45.1
Tests for dissolved oxygen, DO (Winkler method): 18.3.2
Tests for egg age and grading: 9.3
Tests for electric polarity: 11.7.1, potassium chloride with phenolphthalein
Tests for fabrics, Burning tests for fabrics: 4.0.0
Tests for Faraday's first law: 15.1.1.1
Tests for fats and oils: 9.137
Tests for fats and oils, Wijs' solution: 9.137a
Tests for fats proportion in foods: 9.4.5
Tests for food, food tests: 9.8.0
Tests for gases and vapours: 1.0.0
Tests for gases with hot concentrated sulfuric acid: 12.11.3.6
Tests for glucose
Tests for glycerol: 12.12.3
Tests glucose: 19.1.23
Tests for Group 1. Ag, Pb, Hg: 12.11.4.1
Tests for Group 2. Bi, Cd, Cu, Sn: 12.11.4.2
Tests for Group 3. Al, Cr, Fe: 12.11.4.3
Tests for Group 4. Co, Mn, Ni, Zn: 12.11.4.4
Tests for Group 5. Ba, Ca, Sr: 12.11.4.5
Tests for Group 6. K, Mg, Na, NH4: 12.11.4.6
Tests for glycerine: 12.7.4
Tests for gypsum added to the soil: 9.15.5
Tests for haemoglobin, Hematrace test: 9.184
Tests for halides, Cl, Br, I: 12.11.10
Tests for hard water. water hardness: 12.4.0
Tests for hardness of lead, tin, and tin alloys: 3.62.0
Tests for heat
Tests for hydrogen chloride: 3.42.1
Tests for hydrogen gas: 3.41.1.0
Tests for hydrogen peroxide: 17.12.6
Tests for hydrogen sulfide solution: 3.43.1
Tests for hydrolysis of starch, dilute hydrochloric acid: 9.129
Tests for hydrolysis of starch, iodine test, Fehling's solution: 9.10.1
Tests for hydrolysis of starch, salivary amylase: 9.130
Tests for hydrolysis of sucrose, dilute acids: 9.131
Tests for hydroxides: 12.11.11
Tests for hydroxyl ions (hydroxide ions), ammonia solution: 13.6.5
Tests for insoluble solids in rain water: 18.2.1
Tests for iodides: 12.11.12
Tests for ions in a water sample: 18.4.0
Tests for iron: 12.11.3.20
Tests for iron in cooking water: 19.3.3a
Tests for ketones: 19.5.1
Tests for lactic acid solution: 12.7.11
Tests for lead:
Tests for lemon juice effect on apple browning: 19.3.4.2.2 (Cooking)
Tests for lignin: 9.8.21
Tests for limestone: 35.31 (Geology)
Tests for lipase: 9.3.14
Tests for magnesium
Tests for manganese: 12.11.3.23
Tests for metal ions in water, EDTA chelates: 12.13.11
Tests for melting point of lead, tin, and lead-tin alloys: 3.63
Tests for metallic radicals: 12.11.6.0
Tests for metals with borax beads: 12.11.3.1a
Tests for metals with flame tests: 12.11.3.2
Tests for methane gas, burn methane: 16.5.1.2
Tests for milk: 16.0.0
Tests for moisture content of plant organs: 9.3.15
Tests for natural fabrics, burning tests: 4.2.0
Tests for nickel, DMG: 12.11.3.24
Tests for nitrates / nitrites with dipsticks: 19.5.2
Tests for nitrates: 12.11.13
Tests for nitrogen content in food, Kjeldahl method: 16.5.7
Tests for nitrogen content in food, soda lime test: 9.138
Tests for nitrous oxide (dinitrogen oxide), N2O: 3.45.1
Tests for organic compounds: 16.2.9.9
Tests for oxalates: 12.11.14
Tests for oxidase and peroxidase in plant tissues: 9.3.11
Tests for oxidation of glucose, blue bottle experiment: 9.3.7.2
Tests for oxidizing agents:
Tests for oxygen absorption during plant respiration: 9.6.7
Tests for oxygen content of water (dissolved oxygen): 18.3.2
Tests for oxygen gas: 3.49.1
Tests for pectin in jelly and jam: 16.5.8
Tests for pH of the environment: 18.1.0
Tests for phosphate ions in water: 18.4.1
Tests for phosphates: 12.11.15
Tests for phosphorus and phosphates: 12.13.0
Tests for plastics, Burning tests for fabrics: 4.0.0
Tests for polymers: 4.0.0
Tests for potassium, sodium perchlorate: 12.11.3.25
Tests for potassium, tetraphenylborate test: 12.11.3.1.1
Tests for proteins: 9.6.8
Tests for precipitates, acids with lead (II) nitrate: 3.71.4
Tests for precipitates, mix salt solutions: 3.71.3
Tests for rain water (soluble solids): 18.2.3
Tests for reagents, multiple reagent strips: 19.1.20.5
Tests for reducing sugars, Benedict's test
: 9.4.0
Tests for reducing sugars, Fehling's test: 9.5.0
Tests for respiration of soaked peas with limewater, respiration apparatus: 9.155
Test for salt effect on buffer solutions: 12.10.7.4
Tests for salts with flame tests: 12.11.3.7
Tests for salts with flame test sprays: 12.11.3.9
Tests for saturated hydrocarbons, bromine water test: 9.4.7.0
Tests for silver, shining metal: 12.12.11.3.8, (See 8.)
Tests for silver, potassium chromate: 12.11.3.26
Tests for soap: 12.9.2
Tests for sodium: 12.11.3.27
Tests for sodium bicarbonate in a stomach powder: 12.1.20
Tests for sodium chloride, flame test: 12.1.29
Tests for soils, Soil tests: 6.10.0
Tests for solubility: 12.11.3.3
Tests for soluble solids in rain water: 18.2.3
Tests for starch
Tests for strength of mud, clay and sand bricks: 34.65.0
Tests for strength of plaster of Paris bricks: 34.67.0
Tests for strontium: 12.11.3.28
Tests for substances by loss on heating: 12.11.3.1
Tests for substances by sublimation, melting, decrepitation: 12.11.3.4
Tests for substances with dilute hydrochloric acid: 12.11.3.5
Tests for substances with heated charcoal and fusion mixture: 12.11.3.8
Tests for sucrose with Fehling's solution: 9.142.5
Tests for sugars: 9.5.12
Tests for sulfates: 12.11.16
Tests for sulfates in groundwater: 18.2.2.3
Tests for sulfides: 12.11.17
Tests for sulfites: 19.5.3
Tests for sulfur dioxide: 3.51.6
Tests for sulfur in proteins: 9.6.11
Tests for swimming pools: 18.5.0
Tests for synthetic fibres, burning tests: 4.3.0
Tests for tannic acid in tea: 9.8.2a
Tests for tartaric acid: 19.5.4
Tests for thermometer calibration: 22.7.3
Tests for tin: 12.11.3.8 (See: Heat with charcoal 9.)
Tests for tin: 12.11.3.29
Tests for toxic effect of drugs on water flea: 16.5.9
Tests for trimethylamine fish smell: 16.6.1
Tests for turbidity: 16.0
Tests for unknown substances, qualitative analysis: 12.11.3.0
Tests for unsaturation: 9.4.9.0
Tests for urea, urea forms biuret: 16.6.13
Tests for urine: 19.5.5
Tests for vitamin C, DCPIP: 9.143
Tests for water
Tests for water (list): 19.5.7
Tests for water, (in the environment): 18.7.1
Tests for water hardness: 12.4.0
Test wheat starch for gluten: 16.6.2
Tests for wood: 9.144
Tests for zinc: 12.11.3.8, (See: Heat with charcoal 10.)
Tests for zinc: 12.11.3.30
Tests for zymase and catalase in yeast: 9.3.12
Tests with multiple reagent strips: 19.5.6

19.1.23 Tests glucose
19.1.24 Tests for glucose, Clinitest tablet
19.1.25 Tests for glucose, Clinistix strip
19.1.26 Tests for glucose, blood glucose
19.1.26 Tests for glucose concentration, ferricyanide test
19.1.28 Tests for glucose, Nelson-Somogyi test
19.1.29 Tests for glucose, glucose oxidase test
19.1.30 Tests for glucose, glycosylated haemoglobin test
19.1.31 Tests for glucose, urine test
19.1.33 Tests for glucose, urine test, Diastix strip

1.0.0 Tests for gases and vapours
Tests for acetylene, (ethyne): 16.1.3.2
Tests for ammonia: 3.33.1
Tests for carbon dioxide: 3.34.1.1
Tests for carbon dioxide in the breath with limewater : 9.6.10
Tests for carbon monoxide with a gas detector: 18.6.3.1
Tests for chlorine: 3.40.1
Tests for chlorine levels in swimming pools, test kit: 18.7.21.0
Tests for gases collected in a respirometer: 9.6.12
Tests for gases from burning hydrocarbons: 16.4.6.0
Tests for gases with hot concentrated sulfuric acid: 12.11.3.6
Tests for gases: Lighted splint tests
Tests for hydrogen chloride: 3.42.1.0
Tests for hydrogen gas: 3.41.1.0
Tests for hydrogen sulfide solution: 3.43.1
Tests for methane gas, burn methane: 16.5.1.2
Tests for nitrous oxide, dinitrogen oxide, N2O: 3.45.1
Tests for oxygen absorption during plant respiration: 9.6.7
Tests for oxygen gas: 3.49.1
Tests for sulfur dioxide: 3.51.6
Tests for water with cobalt (II) chloride paper: 8.2.2

10.2.9 Tests for ammonia or amino acids or proteins with ninhydrin
Ninhydrin, C9H6O4, indantrione hydrate, (1,2,3-indantrione monohydrate), Toxic by all routes
Ninhydrin, C9H6O4, forms characteristic blue colour on heating.
Spray the paper with a spray reagent of a 2% solution of ninhydrin in ethyl alcohol in an aerosol pack.
Ninhydrin reacts with α-amino acids to yield coloured products.
Leave the filter paper in the fume cupboard, fume hood until the spray solution is dry.
Place the filter paper in an oven at 110oC until all the spots have developed.
Circle the spots with a pencil before they fade.
Measure from the centre of each spot to the base line.
Measure the distance the solvent has travelled at each position and calculate the Rf values for each amino acid.
Find the composition of the unknown by visual comparison of spot colours and by the relationships of Rf values.